Born in Berlin, the mezzo-soprano Stepanka Pucalkova graduated in 2005 studying singing at the High school of Jan Neruda in Prague and 2007 she continued her studies at the University Mozarteum in Salzburg, Austria. There she worked under the guidance of Elisabeth Wilke, Josef Wallnig and Eike Gramss and in the year of 2012 she received her master's degree in opera. The same year Stepanka Pucalkova was awarded by the organization Stiftung the Mozarteum Medal of Lilli Lehmann.
She performed in a number of productions at the Salzburg Mozarteum University and Landestheater in Salzburg, including roles such as Mozart´s Idamante (Idomeneo), 2nd Lady (The Magic Flute) and Marcellina (The Marriage of Figaro), Octavian (R. Strauss: Der Rosenkavalier), Larina ( Tchaikovsky: Eugene Onegin), Galatea (Handel: Aci, Galatea et Polifemo) and Annina (Verdi: La traviata). In summer of 2013 she performed Lehrbube/Die Meistersinger von Nürnberg at the Haus for Mozart (Salzburger Festspiele) with conductor Danielle Gatti and stage director Stefan Herheim. In March 2014 in Salzburg she presented herself as Sesto (Mozart: La clemenza di Tito) and in the same role in Summer of 2014 at Teatro Romano Fiesole in Florenz (Italy)
Worked with famous conductors and directors as Danielle Gatti, Stefan Herheim, Mariusz Trélinski, Mirga Granžynite-Tyla, Friedrich Haider, Marco Guidarini, Jiří Heřman, Leo Hussain, Eike Gramss, Keith Warner and Roland Böer.
In Autumn 2014 Stepanka Pucalkova performed as a guest in the Prague State Opera in the role of the Servant (R. Strauss: Salome), in the Slovak National Theatre in Bratislava (Rosina in Rossini's Barber of Seville and Annius in La clemenza di Tito), as well as Mercedes (Bizet: Carmen) in Landestheater Bregenz und Landesthetare Salzburg.
In season 15/16 the young mezzo made her role debut as Suzuki (G. Puccini: Madama Butterfly), also performed the roles of Stéphano (Ch. Gounod: Roméo et Juliett), 3rd Dame (W.A.Moazrt: The magic flute) and 1. Maidservant (R. Strauss: Elektra) in the State opera Prague. In Autumn 2016 she performed the role of 3rd Lady in „Die Zauberflöte“ on japanese tour with Prague State opera.
In season 16/17 Stepanka performs El Sombrero de tres picos by Manuel De Falla in Kristianand,Siegrune/Die Walküre with Christian Thieleman at Osterfestspiele Salzburg, also Suzuki/Madama Butterfly at State Opera Prague, and 3rd Lady/The Magic Flute on tour in Japan with Prague State Opera.
Future engagements include El Sombrero de tres picos by De Fallain with Kristiansand Symfoniorkester.
In December 2016 she won the second prize in category "The best female voice" on Concours international de Belcanto Vincenzo Bellini" in Marseille.